Open-sourced Quantification pipelines
I’m passionate about developing free, open-source quantitative imaging tools that empower researchers to explore the dynamic world of the cytoskeleton and mitochondria with greater clarity and precision. My goal is to make advanced image analysis accessible to anyone studying how these organelles shape cellular life.
Actin cytoskeleton morphology
Actin cable organization
A set of FIJI macros for automated quantification of actin cable orientation in budding yeast.
Actin cable length and tortuosity
This FIJI macro measures the tortuosity of actin cables in Saccharomyces cerevisiae — quantifying how straight or curved each actin cable is.
Cropping and prepping actin cable movies
This FIJI macro streamlines the process of cropping actin cable movies in Saccharomyces cerevisiae, allowing users to focus on single-cell dynamics within large microscopy fields.
Mitochondrial distribution and quality quantification
Automatic regional quantification (mitochondria/Mfb1)
This FIJI macro quantifies the subcellular distribution of mitochondria or Mfb1 (a mitochondrial-associated protein asymmetrically localized to the mother distal tip in Saccharomyces cerevisiae).
Quantification of ratiometric biosensor
This repository contains scripts to facilitate analysis of mitochondrially targeted ratiometric biosensors using Fiji/ImageJ and R.
I am happy to provide support in customizing our existing imaging pipelines to fit other research questions. I’m excited to help more researchers quantitatively analyze their microscopy data, enabling new discoveries and advancing scientific understanding.